期刊
AQUACULTURE
卷 519, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2019.734882
关键词
Antiviral ability; Lonicera japonica components; Isochlorogenic acid; Caffeic acid; Grouper iridovirus
资金
- National Natural Science Foundation of China [41966004, 41706145]
- Key Research and Development Programs of Guangxi [2018AB52003]
- Natural Science Foundation of Guangxi [2019JJD130019, 2019JJA130194]
- Basic Research Fund of Guangxi Academy of Sciences [2018YJJ903, 2018YJJ902]
- Innovation-driven development special fund project of Guangxi [AA17204044, AA17204095-10]
Grouper iridovirus was isolated from the diseased hybrid grouper in Guangxi province, China (SGIV-Gx), which has a high mortality rate in cultured groupers. Effective medicines for controlling SGIV-Gx infection are urgently needed. Lonicera japonica Thunb. (L. japonica) is a well-known medicinal plant. The application of L. japonica components against SGIV-Gx infection was assessed in this study. The safe, appropriate working concentration of each L. japonica component was identified (L. japonica aqueous extracts LAE <= 10 mg/mL; chlorogenic acid, CGA <= 250 mu g/mL; cryptochlorogenic acid, CCGA <= 500 mu g/mL; isochlorogenic acid A, IAA < 250 mu g/mL; isochlorogenic acid B, IAB <= 250 mu g/mL; isochlorogenic acid C, IAC <= 500 mu g/mL; caffeic acid, CA <= 125 mu g/mL; luteolin (LT) and inositol (IS), no cytotoxic effects even at 2000 mu g/mL) in vitro and in vivo. The inhibitory activities of each L. japonica component against SGIV-Gx infection were analyzed by three methods: light microscopy, Q3-AFMP and RT-qPCR, which proved that, some L. japonica components (LAE, IAA, IAB, IAC, CA, LT, IS) exhibited the antiviral activity against SGIV-Gx infection in a dose-dependent manner, and LAE, IAA, IAB, IAC, CA, LT had the best antiviral activity against SGIV-Gx infection, with above 90% of inhibitory percentage. Overall, the results in this study suggest that L. japonica components have antiviral effects, making this plant a promising candidate for developing effective medicines for preventing and controlling SGIV-Gx infection in farmed groupers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据