期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 104, 期 1, 页码 405-415出版社
SPRINGER
DOI: 10.1007/s00253-019-10286-3
关键词
Loop-mediated isothermal amplification; Naked eye; Droplet digital-based amplification
资金
- WaterChip (IGSTC/BMBF) [FKZ: 01DQ16009A]
- DESPAC (BMBF) [FKZ: 01DH17058]
- RiMaTH (BMBF) [FKZ: 02WRS1276A]
Recently Legionella pneumophila is the main causative waterborne organism of severe respiratory infections. Additionally, other Legionella species are documented as human pathogens. In our work, we describe a rapid detection method which combines two advantages for sensitive and specific detection of the genus Legionella: the fast isothermal amplification method Loop-mediated isothermal AMPlification (LAMP), and a colorimetric detection method using the metal indicator hydroxynaphtol blue (HBN) which allows to determine an optical signal with a simple readout (with the naked eye). Moreover, we present two approaches for minimizing the assay volume using a stationary microchip LAMP and droplet digital-based LAMP (ddLAMP) as promising highly sensitive setups.
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