期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 191, 期 1, 页码 164-176出版社
SPRINGER
DOI: 10.1007/s12010-020-03262-5
关键词
Escherichia coli; LEA peptide; Abiotic stress; Low pH; Late embryogenesis abundant protein
资金
- JSPS KAKENHI [25820405]
- Grants-in-Aid for Scientific Research [25820405] Funding Source: KAKEN
Unsuitable pH is a major limiting factor for all organisms, and a low pH can lead to organism death. Late embryogenesis abundant (LEA) peptides confer tolerance to abiotic stresses including salinity, drought, high and low temperature, and ultraviolet radiation same as the LEA proteins from which they originate. In this study, LEA peptides derived from group 3 LEA proteins of Polypedilum vanderplanki were used to enhance low pH tolerance. Recombinant Escherichia coli BL21 (DE3) cells expressing the five designed LEA peptides were grown at pH 4, 3, and 2. The transformants showed higher growth capacity at low pH as compared to control cells. These results indicate that LEA peptide could prevent E. coli cell death under low pH conditions.
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