期刊
ANTICANCER RESEARCH
卷 40, 期 2, 页码 689-694出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.13998
关键词
Apoptosis; breast cancer; methoxy poly(ethylene glycol)-poly(lactic-co-glycolic) acid; piperine; thin-film hydration
类别
资金
- Canadian Cancer Society/Canadian Breast Cancer Foundation Endowed Chair in Breast Cancer Research
- Nova Scotia Graduate Scholarship
- Cancer Research Training Program Trainee Award
Background/Aim: Piperine, a major alkaloid of the fruit of black pepper plants, selectively inhibits the growth of triple-negative breast cancer cells but its lipophilicity restricts possible clinical application. This study therefore determined the feasibility of encapsulating piperine in nanoparticles (NPs) to increase its solubility in an aqueous environment. Materials and Methods: Piperine-loaded biodegradable methoxy poly(ethylene glycol)poly(lactic-co-glycolic) acid copolymer-based NPs were produced by single emulsion solvent extraction and thin-film hydration. Growth and viability of triple-negative breast cancer (TNBC) cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Annexin-V-FLUOS/propidium iodide staining, respectively. Results: Thin-film hydration was superior to single emulsion solvent extraction, yielding piperine-loaded NPs with an average size of 50 nm. Piperine-loaded NPs inhibited TNBC cell growth and induced apoptosis while sparing normal fibroblasts. Conclusion: It is feasible to deliver a cytotoxic concentration of piperine to TNBC cells via NPs with the potential for improved bioavailability and solubility in biological fluids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据