期刊
BIORESOURCE TECHNOLOGY
卷 188, 期 -, 页码 240-246出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.01.048
关键词
Cellulase; Penicillium; Biomass; Bioreactor; Submerged fermentation
资金
- DBT
- Indian Oil Corporation R & D Centre and Department of Biotechnology, Govt. of India
Present study was focused on cellulase production from an indigenously isolated filamentous fungal strain, identified as Penicillium oxalicum. Initially, cellulase production under submerged fermentation in shake flasks resulted in cellulase activity of 0.7 FPU/mL. Optimization of process parameters enhanced cellulase production by 1.7-fold and resulted in maximum cellulase activity of 1.2 FPU/mL in 8 days. Cellulase production was successfully scaled-up to 7 L fermenter under controlled conditions and incubation time was reduced from 8 days to 4 days for achieving similar cellulase titer. Optimum pH and temperature for activity of the crude enzyme were pH 5 and 50 degrees C, respectively. At 50 degrees C the produced cellulase retained approximately 50% and 26% of its activity at 48 h and 72 h, respectively. Hydrolytic efficiency of P. oxalicum was comparable to commercial cellulase preparations which indicate its great potential for application in the lignocellulose hydrolysis. (C) 2015 Elsevier Ltd. All rights reserved.
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