期刊
ANIMAL BIOTECHNOLOGY
卷 32, 期 5, 页码 544-549出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2020.1726363
关键词
SIRT3; Malabari; Attappady Black; ovarian expression; SNP; litter size
资金
- Kerala Veterinary and Animal Sciences University, Kerala, India
- AICRP on Malabari Goat Improvement Scheme (ICAR)
This study investigated the role of SIRT3 gene in goat fertility, finding higher expression of SIRT3 in Malabari goats, polymorphism in exon 7, and significant association between SIRT3 genotypes and litter size, suggesting SIRT3 as a potential candidate gene for genetic improvement in goats.
The NAD(+)-dependent protein deacetylase, Sirtuin3 (SIRT3), plays a role in fertility by preventing the activation of reactive oxygen species (ROS). A novel study was conducted on caprine SIRT3, to study its ovarian expression, explore the sequence variability in exon 7 and analyze its association with prolificacy in two native goat breeds of Kerala, Malabari and Attappady Black. The mRNA isolated from ovaries of six Malabari and Attappady Black goats were subjected to quantitative PCR (qPCR) using GAPDH and beta-actin as reference genes. Genomic DNA was isolated from 185 goats (99 Malabari and 86 Attappady Black) and subjected to PCR-SSCP to identify polymorphism in exon 7 of SIRT3 and association with litter size was analyzed. The ovarian expression of caprine SIRT3 was significantly higher (p <= 0.01) in Malabari than low prolific Attappady Black. PCR-SSCP analysis revealed, exon 7 of SIRT3 was polymorphic with three genotypes namely, AA, AB and BB with a novel SNP, g.154C > T in the 3 ' UTR. A significant association (p <= 0.05) was noticed between the genotypes of SIRT3 and litter size. The results obtained from this study highlight the role of SIRT3 in reproduction and hence SIRT3 may be considered as a potential candidate gene for genetic improvement in goats.
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