期刊
ANIMAL BIOTECHNOLOGY
卷 32, 期 3, 页码 318-326出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10495398.2019.1691010
关键词
FGF21; intramuscular adipocyte; differentiation; KLFs
资金
- National Key R&D Program of China [2018YFD0502002]
- Applied Basic Research Program Key Project of Sichuan Province [2018JY0036]
- 13th Five-Year' livestock and poultry breeding project of Sichuan Province [2016NYZ0045]
- Collaborative Innovation Center for Ecological Animal Husbandry of Qinghai-Tibetan Plateau Open Foundation [QZGYXT01]
The study demonstrates that FGF21 plays a key role in the regulation of adipogenic differentiation in goat intramuscular preadipocyte by affecting the expression of various genes and having a negative impact on lipid accumulation.
Fibroblast growth factor 21 (FGF21) plays a critical role in the regulation of lipid metabolism; however, its function in goat intramuscular fat (IMF) deposition remains unknown. To explore the role of FGF21 for goat IMF deposition, we performed gain and loss function of FGF21 in intramuscular adipocyte. Our results showed that overexpression of FGF21 mediated by adenovirus inhibits lipid accumulation of goat intramuscular adipocyte, accompanied by down-regulating the mRNA levels of peroxisome proliferator activated receptor gamma (PPAR gamma), adipocyte fatty acid-binding protein 2 (aP2) and sterol regulatory element-binding protein 1 (SREBP1), but up-regulating counterpart of preadipocyte factor1 (Pref1). Conversely, siRNAs knocking down FGF21 promotes the expression of PPAR gamma and CCAAT/enhancer binding protein-alpha (C/EBP alpha) but suppressed that of lipoprotein lipase (LPL) and Pref1. Meanwhile, we found that FGF21 regulates the expression of many KLFs transcription factors, such as KLF3, 7, 9, 11, 14, and 16. These findings demonstrate a key role of FGF21 as a negative factor in the regulation of adipogenic differentiation in goat intramuscular preadipocyte.
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