期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 15, 页码 6192-6195出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201914449
关键词
gene expression; LuxRs; natural products; quorum sensing; transcriptional activation
资金
- Leibniz Association
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [390713860]
- collaborative research cluster ChemBioSys SFB1127
- Exploration Grant of the Boehringer Ingelheim Foundation (BIS)
- Europaischer Fonds fur regionale Entwicklung (EFRE)
- DECHEMA
LuxR-type transcriptional activator proteins frequently regulate the expression of biosynthetic gene clusters (BGCs). With only a fraction of bacterial BGCs being expressed under standard culturing conditions, modulation of LuxRs would provide a powerful approach to activate silent clusters. We show that by exploiting the modular nature of LuxR proteins, it is possible to construct functional chimeric LuxRs, which enables both the rewiring of quorum sensing systems and the activation of silent BGCs. Importantly, our strategy allowed us to identify the novel natural product pseudomonol from a bacterium of the genus Pseudomonas.
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