期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 13, 页码 5308-5311出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201914338
关键词
acylation; chitin de-N-acetylases; enzymatic synthesis; glycosylation; unnatural sialosides
资金
- BBSRC [BB/M029034/1, BB/M028836/1]
- EPSRC [BB/M029034/1, BB/M028836/1]
- InnovateUK: IBCatalyst programme [BB/M029034/1, BB/M028836/1]
- Marie Sklodowska-Curie Innovative Training Network [H2020-MSCA-ITN-2014-ETN-642870]
- European Research Council (ERC) [ProgrES-DVL788231]
- National Natural Science Foundation of China (NSFC) [31471703, 31671854, 31871793, 31871754]
- BBSRC [BB/M029034/1, BB/M028836/1] Funding Source: UKRI
Chitin is one of the most abundant and cheaply available biopolymers in Nature. Chitin has become a valuable starting material for many biotechnological products through manipulation of its N-acetyl functionality, which can be cleaved under mild conditions using the enzyme family of de-N-acetylases. However, the chemoselective enzymatic re-acylation of glucosamine derivatives, which can introduce new stable functionalities into chitin derivatives, is much less explored. Herein we describe an acylase (CmCDA from Cyclobacterium marinum) that catalyzes the N-acylation of glycosamine with a range of carboxylic acids under physiological reaction conditions. This biocatalyst closes an important gap in allowing the conversion of chitin into complex glycosides, such as C5-modified sialosides, through the use of highly selective enzyme cascades.
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