期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 13, 页码 5293-5297出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201914955
关键词
aptamers; G-quadruplexes; nucleic acids; RNA; RNA structures
资金
- Research Grants Council of the Hong Kong SAR, China [CityU 11101519, CityU 11100218, N_CityU110/17, CityU 21302317]
- Croucher Foundation [9500030, 9500039]
- Shenzhen Basic Research Project [JCYJ20180507181642811]
G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first l-RNA aptamer that targets a d-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4-RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.
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