期刊
ANALYTICAL CHEMISTRY
卷 92, 期 1, 页码 1122-1129出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04188
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资金
- Ministere de l'Enseignement Superieur, de la Recherche et de l'Innovation (MESRI)
- Institut National de la Sante et de la Recherche Medicale (Inserm)
- Universite de Lille
Large scale proteomic strategies rely on database interrogation. Thus, only referenced proteins can be identified. Recently, Alternative Proteins (AltProts) translated from nonannotated Alternative Open reading frame (AltORFs) were discovered using customized databases. Because of their small size which confers them peptide-like physicochemical properties, they are more difficult to detect using standard proteomics strategies. In this study, we tested different preparation workflows for improving the identification of AltProts in NCH82 human glioma cell line. The highest number of identified AltProts was achieved with RIPA buffer or boiling water extraction followed by acetic acid precipitation.
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