期刊
ANALYTICAL CHEMISTRY
卷 92, 期 1, 页码 1395-1401出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04573
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资金
- National Natural Science Foundation of China [21922402, 21874017, 21727811, 21605014]
- Liaoning Provincial Program for Promoting Talents [XLYC1807005, XLYC1802016]
- Fundamental Research Funds for the Central Universities [N180504003, N182410008-1]
Longitudinal surface plasmon resonance (LSPR)-based optical signals possess unique advantages in biomolecular sensing and detection which can be attributed to their ultrahigh sensitivity and signal-to-noise ratio. However, the lack of effective strategies for morphological control of gold nanorods (GNRs) complicates the precise tuning of their LSPR property. Herein, a peptide-encoded strategy was first developed to precisely control the morphologies of GNRs via overgrowth of GNR seeds in the presence of thiol-containing peptides. Significantly, the peptide-encoded GNRs exhibit a tunable LSPR peak ranging from 685 to 877 nm by altering the amount of peptide. A few obvious colorimetric changes were accompanied from pink to purple and even to blue. Other parameters, e.g., pH, temperature, and Ag+ concentration, could also be utilized to regulate the morphologies of the peptide-encoded GNRs. The ultrasensitive detection of tumor-related protease activities based on LSPR peak shifts was further successfully performed without the need for labeling or instrumental aid, achieving a limit of detection of 60 fM. It is much lower than traditional absorption-based analysis (1 nM) and enzyme-linked immunosorbent assay (ELISA) method (1 pM), indicating the great potential of this peptide-encoded strategy in the application of ultrasensitive biomarker assay and clinical diagnosis.
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