期刊
ANALYTICAL CHEMISTRY
卷 92, 期 4, 页码 3354-3360出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b05372
关键词
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资金
- National Postdoctoral Program for Innovative Talents [BX20180117]
- Jilin Province Science and Technology Development Program [20190201233JC]
- National Key Research and Development Program of China [2016YFA0200400]
- National Natural Science Foundation of China [51571100, 51872116, 51702116, 51602305, 31771093]
- Program for JLU Science and Technology Innovative Research Team (JLUSTIRT) [2017TD-09, 2017TD-27, 2019TD-36]
- Project of International Collaboration of Jilin Province [201180414085GH]
Herein, we report on a two-dimensional amino-functionalized Ti3C2-MXene (N-Ti3C2-MXene)-based surface plasmon resonance (SPR) biosensor for detecting carcinoembryonic antigen (CEA) utilizing a sandwich format signal amplification strategy. Our biosensor employs an N-Ti3C2-MXene nanosheet-modified sensing platform and a signal enhancer comprising N-Ti3C2-MXene-hollow gold nanoparticles (HGNPs)-staphylococcal protein A (SPA) complexes. Ultrathin Ti3C2-MXene nanosheets were synthesized and functionalized with aminosilane to provide a hydrophilic-biocompatible nanoplatform for covalent immobilization of the monoclonal anti-CEA capture antibody (Ab(1)). The N-Ti3C2-MXene/HGNPs nanohybrids were synthesized and further decorated with SPA to immobilize the polyclonal anti-CEA detection antibody (Ab(2)) and serve as signal enhancers. The capture of CEA followed by the formation of the Ab(2)-conjugated SPA/HGNPs/N-Ti3C2-MXene sandwiched nanocomplex on the SPR chip results in the generation of a response signal. The fabricated N-Ti3C2-MXene-based SPR biosensor exhibited a linear detection range of 0.001-1000 PM with a detection limit of 0.15 fM. The proposed biosensor showed high sensitivity and specificity for CEA in serum samples, which gives it application potential in the early diagnosis and monitoring of cancer. We believe that this work also opens new avenues for development of MXene-based highly sensitive biosensors for determining various biomolecules.
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