4.5 Article

Development of a new chromogenic method for putrescine quantification using coupling reactions involving putrescine oxidase

期刊

ANALYTICAL BIOCHEMISTRY
卷 593, 期 -, 页码 -

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ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2020.113607

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  1. Institute for Fermentation, Osaka [K-25-04]
  2. JSPS KAKENHI [16K14905, 17H05026]
  3. Grants-in-Aid for Scientific Research [16K14905, 17H05026] Funding Source: KAKEN

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Quantification of polyamines, including putrescine, is generally performed using high-performance liquid chromatography (HPLC) or gas chromatography. However, these methods are time-consuming because of sample derivatization and analytical reagent preparation. In this study, we developed a simple and high-throughput putrescine quantification method on a 96-well microtiter plate using putrescine oxidase from Rhodococcus erythropolis NCIMB 11540, peroxidase, 4-aminoantipyrine, and N-ethyl-N-(3 sulfopropyl)-3-methylaniline sodium salt. The developed method (named as PuO-POD-4AA-TOPS method) was applicable to bacterial culture supernatants. Furthermore, putrescine concentrations determined by the developed method roughly corresponded to the concentrations determined by HPLC.

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