期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 412, 期 11, 页码 2579-2587出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-02484-0
关键词
Ru(II) complex; Metallopolymer; Oxygen nanosensor; Hypoxia imaging
资金
- Natural Science Foundation of China [61775245]
- National Key R&D Program of China [2017YFB0405400]
- MUC 111 project
A series of Ru(II)-containing metallopolymers with different polypyridyl complexes, namely [Ru(N<^>N)(2)(L)](PF6)(2) (L = bipyridine-branched polymer; N<^>N = bpy: 2,2 '-bipyridine (Ru 1); phen: 1,10-phenanthroline (Ru 2); dpp: 4,7-diphenyl-1,10-phenanthroline (Ru 3)), were synthesized with the motive that adjusting pi-conjugation length of ligands might produce competent luminescent oxygen probes. The three hydrophobic metallopolymers were studied with H-1 NMR, UV-Vis absorption, and emission spectroscopy, and then were utilized to prepare biocompatible nanoparticles (NPs) via a nanoprecipitation method. Luminescent properties of the NPs were investigated against dissolved oxygen by steady-state and time-resolved spectroscopy respectively. Luminescence quenching of the three NPs all followed a linear behavior in the range of 0-43 ppm (oxygen concentration), but Ru 3-NPs exhibited the highest oxygen sensitivity (82%) and longest emission wavelength (lambda(ex) = 460 nm; lambda(em) = 617 nm). In addition, external interferons from cellular environments (e.g., pH, temperature, and proteins) had been studied on Ru 3-NPs. Finally, dissolved oxygen in monolayer cells under normoxic/hypoxic conditions was clearly differentiated by using Ru 3-NPs as the luminescent sensor, and, more importantly, hypoxia within multicellular tumor spheroids was vividly imaged. These results suggest that such Ru(II)-containing metallopolymers are strong candidates for luminescent nanosensors towards hypoxia.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据