期刊
ANALYTICA CHIMICA ACTA
卷 1103, 期 -, 页码 97-105出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.12.052
关键词
Aptamer; Molecularly imprinted polymer; Ochratoxin A; Monolithic column; Double recognition
资金
- National Natural Science Foundation of China [21307013]
- Science and Technology program of Fujian Province [2019Y0003]
- Natural Science Foundation of Fujian Province [2015J01044]
In this study, a double-recognized aptamer-molecularly imprinted monolithic column (Apt-MIP monolithic column) was prepared by introducing both aptamer and MIP to reduce non-specific adsorption. Its preparation parameters such as the time of photo-initiation, the dosage of photo-initiator and the concentration of aptamer were investigated in detail. The recovery ratios of ochratoxin A (OTA) to ochratoxin B (OTB) on Apt-MIP monolithic column, Apt monolithic column and MIP monolithic column were 116.1, 40.8 and 69, respectively. Even if the concentration of OTB was 10 times that of OTA, the recovery of OTB was only about 2.9%. Applied to beer samples, the prepared Apt-MIP monolithic column drastically resisted background adsorption and the high-specificity recognition for OTA was obtained with the recoveries of 95.5-105.9%. This work provided a simple and effective method to selectively identify OTA from complex samples. (c) 2019 Elsevier B.V. All rights reserved.
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