期刊
ANALYTICA CHIMICA ACTA
卷 1102, 期 -, 页码 119-129出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.12.050
关键词
Gold nanoparticle; Spherical nucleic acid; Palindromic linker; Colorimetric detection; HIV-1 nucleic acid; Phosphorothioate modification
Gold nanoparticle-core spherical nucleic acids (AuNP core-SNAs), by virtue of the programmable nature of oligonucleotides, have yielded access to the innovative strategies for targeted biodiagnostics. Here, DNA-directed self-assembly of AuNP core-SNAs has been used to design a colorimetric method to sense HIV-1 viral nucleic acid. This strategy utilizes an oligonucleotide with sequence of 5'-untranslated region (5' UTR) of the HIV-1 RNA genome anchored on the surface of AuNPs and a complementary linker strand with a palindromic sequence tail. In the absence of HIV-1 target nucleic acid the complementary linker induces self-assembly of SNAs based on sequence symmetry in the free palindromic tail which can bridge two DNA double helices. While in the presence of the target DNA, due to linker-target duplex formation, the colloidal stability and the red color of the SNAs solution are preserved. Picomole amounts of target DNA can easily be detected with the naked eyes. A 95-mer synthetic DNA strand with the same sequence of HIV-1 viral RNA was utilized for positive control of HIV-1 RNA. The selectivity of the selected linker was satisfactory up to 90% match. (C) 2019 Elsevier B.V. All rights reserved.
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