期刊
ANALYTICA CHIMICA ACTA
卷 1106, 期 -, 页码 52-60出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.01.061
关键词
SUPRAS; Microextraction; Tricyclic antidepressants; Paper spray ionization; Urine
资金
- Coordenacao de Aperfeicoamento de Nivel Superior (CAPES) [Pro-Forenses 3353/2014, 88882.160479/2014e01, 88887.162829/2018e00]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [481669/2013-2, 305552/2013-9, 311502/2016-4, 307432/2017-3]
- Fundacao Araucaria [163/2014]
- Fundacao de Amparoa Pesquisa do Estado de Minas Gerais [RED-00042-16]
- Instituto Nacional de Ciencia e Tecnologia de Bioanalitica (INCT) [573672/2008-3]
- Universidade Estadual de Londrina (UEL)
- Universidade Federal de Minas Gerais (UFMG)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Brasil (CAPES) [001]
This work describes a novel methodology to analyze four tricyclic antidepressants (amitriptyline, doxepin, imipramine and, nortriptyline) in urine samples by combining supramolecular microextraction and paper spray ionization mass spectrometry (PS-MS). The proposed method uses a supramolecular solvent in which reverse micelles of 1-decanol are dispersed in tetrahydrofuran (THF)/water. The extraction of the tricyclic antidepressants at pH 9.0 requires a sample volume of 10.0 mL, short extraction time (1.0 min of extraction and 5 min of centrifugation), low amounts of organic solvent (50 mu L of 1-decanol and 200 mu L of THF), and provides high preconcentration factors: 96.9 to amitriptyline, 93.6 to doxepin, 71.3 to imipramine, and 146.9 to nortriptyline. The quantification by PS-MS is fast and straightforward because chromatographic separation is not required and all analytes were determined simultaneously. The limits of detection (LOD), quantification (LOQ), and the precision (RSD, %) of the developed method ranged between 5.2 and 8.6 mu g L-1, 17.4-28.7 mu g L-1 and 1.3-12.9%, respectively. Urine samples of five individuals (three males and two females) were used for accuracy evaluation. The accuracy obtained in these spiked urine samples at mu g L-1 levels varied from 95.3 to 112.0%. The method also provided clean mass spectra with a high signal-to-noise ratio, which demonstrates the analytical appeal combination of supramolecular microextraction with determination by paper spray mass spectrometry. (C) 2020 Elsevier B.V. All rights reserved.
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