期刊
ANALYTICA CHIMICA ACTA
卷 1108, 期 -, 页码 177-197出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.01.069
关键词
Convective PCR (CPCR); Thermal convection; Pseudo-isothermal; Nucleic acid diagnosis; Point-of-care test (POCT)
资金
- National Natural Science Foundation of China [81871505, 81371711, 61571420, 61971026]
- National Science and Technology Major Project [2018ZX10732101-001-009]
- Fundamental Research Funds for the Central Universities [XK1802-4, PYBZ1830, PT1908]
- Beijing University of Chemical Technology [buctylkjcx06]
Polymerase chain reaction (PCR) is an extremely important tool for molecular diagnosis, as it can specifically amplify nucleic acid templates for sensitive detection. As another division of PCR, free convective PCR was invented in 2001, which can be performed in a capillary tube pseudo-isothermally within a significantly short time. Convective PCR thermal cycling is implemented by inducing thermal convection inside the capillary tube, which stratifies the reaction into spatially separate and stable melting, annealing, and extension zones created by the temperature gradient. Convective PCR is a promising tool that can be used for nucleic acid diagnosis as a point-of-care test (POCT) due to the significantly simplified heating strategy, reduced cost, and shortened detection time without sacrificing sensitivity and accuracy. Here, we review the history of free convective PCR from its invention to development and its commercial applications. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据