期刊
ANALYTICA CHIMICA ACTA
卷 1094, 期 -, 页码 122-129出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.10.004
关键词
Fluorescent probe; Hypochlorite acid; Lysosome; Coumarin; Imaging
资金
- Research Project of Department of Education of Guangdong Province [2017GkQNCX003]
- Science and Technology Planning Project of Guangzhou City [201904010250]
- fund of Specialized Cultivation of Scientific and Technological Innovation for College Students in Guangdong Province [pdjh2019b0691]
- Science and Technology Planning Project of Shenzhen City [JCYJ20180307164055935]
- Hunan Provincial Engineering Research Center for Food Processing of Aquatic Biotic Resources [2018KJY06]
- National Key R&D Program of China [2017YFC1600306]
Hypochlorite (HClO) is involved in various physiological and pathological processes as well as regulation of lysosomal functions. Thus, it is appreciated to develop efficient molecule tools for precisely detecting HClO in lysosomes. Although several lysosomal-targetable fluorogenic probes for HClO have been developed to date, they still suffered from the discounted sensing performance under lysosomal acidic condition. Herein, on the basis of the AND logic gate strategy, a novel dual-activatable fluorogenic probe CS has been rationally designed by simultaneously incorporating HClO recognition site and pH-sensitive group with lysosomal-targetable characteristic into a coumarin fluorophore. Different from the single-activated ones previously reported, CS exhibited good sensitivity, high specificity and fast response towards HClO under the acidic conditions but was out of operation in the neutral or alkaline environment. Importantly, it had been successfully applied for spatial-resolution imaging of exogenous or endogenous HClO in lysosomes. (C) 2019 Published by Elsevier B.V.
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