期刊
ANALYTICA CHIMICA ACTA
卷 1101, 期 -, 页码 129-134出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.12.018
关键词
Carbon dots; Fluorescent nanoprobe; Inner filter effect; N-acetyl-beta-D-glucosaminidase; Human serum
资金
- Fundamental Research Funds for the Central Universities, China [2662018JC011, 2662019PY023]
Carbon dots (CDs) were synthesized from p-aminophenol and ethylenediamine via one-step under mild condition and used as a sensitive fluorescent nanoprobe for the activity determination of N-acetyl-beta-D-glucosaminidase (NAG). In this assay, p-nitrophenol was in situ produced from p-nitrophenyl beta-D-N acetyl-glucosaminide, which was exclusively hydrolyzed by NAG. The UV absorption peak of p-nitrophenol (maximum at 400 nm) overlapped the excitation peak of CDs with maximum wavelength at 415 nm, which caused the fluorescence decline of CDs based on inner filter effect. The activity of NAG was determined by the fluorescence changes. The assay is highly sensitive to NAG with a low detection limit of 0.75 U L-1 (K = 3) and showed a good linear relationship in the range from 1 to 45 U L-1. This CDs nanoprobe was successfully applied for the determination of NAG activity in human serum and urine samples. (C) 2019 Elsevier B.V. All rights reserved.
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