期刊
AMERICAN JOURNAL OF TRANSPLANTATION
卷 20, 期 5, 页码 1431-1438出版社
WILEY
DOI: 10.1111/ajt.15737
关键词
B cell biology; basic (laboratory) research; science; heart (allograft) function; dysfunction; heart transplantation; cardiology; immunobiology; rejection; chronic
资金
- National Institute of Allergy and Infectious Diseases [AI116814]
Cardiac allograft vasculopathy (CAV) is associated with intragraft B cell infiltrates. Here, we studied the clonal composition of B cell infiltrates using 4 graft specimens with CAV. Using deep sequencing, we analyzed the immunoglobulin heavy chain variable region repertoire in both graft and blood. Results showed robust B cell clonal expansion in the graft but not in the blood for all cases. Several expanded B cell clones, characterized by their uniquely rearranged complementarity-determining region 3, were detected in different locations in the graft. Sequences from intragraft B cells also showed elevated levels of mutated rearrangements in the graft compared to blood B cells. The number of somatic mutations per rearrangement was also higher in the graft than in the blood, suggesting that B cells continued maturing in situ. Overall, our studies demonstrated B cell clonal expansion in human cardiac allografts with CAV. This local B cell response may contribute to the pathophysiology of CAV through a mechanism that needs to be identified.
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