期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 318, 期 4, 页码 L684-L697出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00128.2019
关键词
collagen; fibroblasts; fibrosis; myofibroblasts; transdifferentiation
资金
- Austrian Science Foundation (FWF) [P 27848-B28, T1032-B34]
- Jubilee Foundation of the Austrian National Bank [16187]
- Deutsche Forschungsgemeinschaft [CRC1213]
- European Respiratory Society Long Term Fellowship [LTRF201801-00308]
Pulmonary fibrosis is characterized by pronounced collagen deposition and myofibroblast expansion, whose origin and plasticity remain elusive. We utilized a fate-mapping approach to investigate a-smooth muscle actin (alpha SMA)+ and platelet-derived growth factor receptor a (PDGFR alpha)+ cells in two lung fibrosis models, complemented by cell type-specific next-generation sequencing and investigations on human lungs. Our data revealed that alpha SMA+ and PDGFR alpha+ cells mark two distinct mesenchymal lineages with minimal transdifferentiation potential during lung fibrotic remodeling. Parenchymal and perivascular fibrotic regions were populated predominantly with PDGFR alpha+ cells expressing collagen, while alpha SMA+ cells in the parenchyma and vessel wall showed variable expression of collagen and the contractile protein desmin. The distinct gene expression profile found in normal conditions was retained during pathologic remodeling. Cumulatively, our findings identify alpha SMA+ and PDGFR alpha+ cells as two separate lineages with distinct gene expression profiles in adult lungs. This cellular heterogeneity suggests that anti-fibrotic therapy should target diverse cell populations.
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