期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 38, 期 7, 页码 1365-1372出版社
SPRINGER
DOI: 10.1007/s00449-015-1377-8
关键词
Modification; Sugarcane bagasse; Butanol; Clostridium acetobutylicum XY16; Polyethylenimine
资金
- 973 Program of China [2011CB707405]
- National Natural Science Foundation of China [21106067]
- State Key Laboratory of Materials-Oriented Chemical Engineering Foundation of Nanjing University of Technology [ZK201001]
- Program for New Century Excellent Talents in University
- Program for Changjiang Scholars and Innovative Research Team in University [06-A-047]
- Jiangsu Province Natural Science Foundation for Youth [BK20140940]
Sugarcane bagasse was chemically modified by polyethylenimine (PEI) and glutaraldehyde (GA) and then used as a support to immobilize Clostridium acetobutylicum XY16 in the process of butanol production. Compared with batch fermentation using unmodified sugarcane bagasse, 22.3 g/L total solvents were produced by cells immobilized on 4 g/L PEI treated sugarcane bagasse with high solvent productivity of 0.62 g/(L h) and glucose consumption rate of 1.67 g/(L h). Improvement of 14, 43, and 37 % in total solvent titer, solvent productivity and glucose consumption rate was observed, respectively. Enhanced solvent production of 25.14 g/L was obtained when using a high concentration of glucose of 80 g/L. Continuous fermentation was studied using PEI/GA modified sugarcane bagasse as immobilization support with a range of dilution which rates from 0.2 to 2.5 to find an optimal condition. The maximum solvent productivity of 11.32 g/(L h) was obtained at a high dilution rate of 2.0 h(-1).
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