期刊
BMC GENOMICS
卷 17, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12864-016-2612-7
关键词
Campylobacter; Chicken; Resistance; Back-cross; Advanced intercross; Genome-wide association; Quantitative trait
资金
- Department for the Environment, Food and Rural Affairs (LINK grant) [LK0665]
- European Union [SABRE - FOOD-CT-2006-01625]
- EADGENE
- Biotechnology and Biological Sciences Research Council (Core Strategic Grant to The Pirbright Institute)
- Scottish Government via the Strategic Partnership for Animal Science Excellence initiative
- Biotechnology and Biological Sciences Research Council (Core Strategic Grant to Roslin Institute)
- Biotechnology and Biological Sciences Research Council (Core Strategic Grant to BBSRC-Aviagen LINK grant) [BB/J006815/1]
- BBSRC [BBS/E/D/20320000, BBS/E/D/20231760, BBS/E/I/00001505, BB/J006815/1, BB/J016837/1, BBS/E/I/00001712] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J006815/1, BBS/E/I/00001712, BBS/E/D/20231760, BBS/E/I/00001505, BBS/E/D/20320000, BB/J016837/1] Funding Source: researchfish
Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(6(1) x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9th generation advanced intercross (6(1) x N) line. Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results. Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.
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