期刊
AGING-US
卷 11, 期 24, 页码 11988-12001出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.102524
关键词
circRNA; fracture healing; miRNA
资金
- National Science Foundation of China [81772345]
- Ministry of Science and Technology of the People's Republic of China [2018YF2001502, 2018YFB1105705]
- Wuhan Science and Technology Bureau [2017060201010192]
- National Health Commission of the People's Republic of China [ZX-01-018, ZX-01-C2016153]
- Health Commission of Hubei Province [WJ2019Z009]
Fracture healing is a complex process involving various cell types, cytokines, and mRNAs. Here, we report the roles of the circRNA AFF4/miR-7223-5p/PIK3R1 axis during fracture healing. We found that increased expression of PIK3R1 during fracture healing is directly associated with augmented proliferation and decreased apoptosis of MC3T3-E1 cells. Furthermore, miR-7223-5p targeted PI3KR1 and inhibited MC3T3-E1 proliferation while promoting apoptosis. CircRNA AFF4 acted as a sponge of miR-7223-5p, thereby promoting MC3T3-E1 cell proliferation and inhibiting apoptosis. Local injection of circRNA AFF4 into femoral fracture sites promoted fracture healing in vivo while the injection of miR-7223-5p delayed healing. These findings suggest that CircRNA AFF4 promotes fracture healing by targeting the miR-7223-5p/PIK3R1 axis, and suggests miR-7223-5p, CircRNA AFF4, and the miR-7223-5p/PIK3R1 axis are potential therapeutic targets for improving fracture healing.
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