期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 39, 期 1, 页码 75-80出版社
SPRINGER
DOI: 10.1007/s00449-015-1491-7
关键词
Ganoderic acid production; Ganoderma lucidum; Bioprocessing strategy; Medicinal mushroom fermentation; Bioactive secondary metabolites
资金
- National Natural Science Foundation of China [31360495, 21566016]
- Kunming University of Science and Technology [KKSY201226107]
- National High Technology R&D Program (863 project) [2012AA021701]
To further improve the ganoderic acid (GA) production, a novel integrated strategy by combining nitrogen limitation and calcium ion addition was developed. The effects of the integrated combination on the content of GA-T (one powerful anticancer compound), their intermediates (squalene and lanosterol) and on the transcription levels of GA biosynthetic genes in G. lucidum fermentation were investigated. The maximum GA-T content with the integrated strategy were 1.87 mg/ 100 mg dry cell weight, which was 2.1-4.2 fold higher than that obtained with either calcium ion addition or nitrogen limitation alone, and it is also the highest record as ever reported in submerged fermentation of G. lucidum. The squalene content was increased by 3.9- and 2.2-fold in this case compared with either individual strategy alone. Moreover, the transcription levels of the GA biosynthetic genes encoding 3-hydroxy-3-methyglutaryl coenzyme A reductase and lanosterol synthase were also up-regulated by 3.3-7.5 and 1.3-2.3 fold, respectively.
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