期刊
ADVANCED MATERIALS
卷 32, 期 7, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201905914
关键词
enzymes; hydrogels; liposomes; microbubbles; ultrasound
类别
资金
- Ermenegildo Zegna Founder's Scholarship program
- Rosetrees Trust
- Arthritis Research U.K. Foundation [21138]
- Medical Research Council [MR/S00551X/1]
- Whitaker International Program, Institute of International Education, United States of America
- UK Regenerative Medicine Platform Acellular Approaches for Therapeutic Delivery [MR/K026682/1]
- UK Regenerative Medicine Platform Acellular/Smart Materials - 3D Architecture [MR/R015651/1]
- Engineering and Physical Science Research Council (EPSRC) grant Bio-functionalised nanomaterials for ultrasensitive biosensing [EP/K020641/1]
- British Heart Foundation Centre of Research Excellence [RE/13/4/30184]
- Science and Technology Facilities Council [RB1810203]
- NSF [DMR-0520547]
- European Union's Horizon 2020 research and innovation programme under the SINE2020 project [654000]
- EPSRC [EP/K020641/1] Funding Source: UKRI
- MRC [MR/S00551X/1, MR/R015651/1, MR/K026666/1, MR/K026682/1] Funding Source: UKRI
Hydrogels are formed using various triggers, including light irradiation, pH adjustment, heating, cooling, or chemical addition. Here, a new method for forming hydrogels is introduced: ultrasound-triggered enzymatic gelation. Specifically, ultrasound is used as a stimulus to liberate liposomal calcium ions, which then trigger the enzymatic activity of transglutaminase. The activated enzyme catalyzes the formation of fibrinogen hydrogels through covalent intermolecular crosslinking. The catalysis and gelation processes are monitored in real time and both the enzyme kinetics and final hydrogel properties are controlled by varying the initial ultrasound exposure time. This technology is extended to microbubble-liposome conjugates, which exhibit a stronger response to the applied acoustic field and are also used for ultrasound-triggered enzymatic hydrogelation. To the best of the knowledge, these results are the first instance in which ultrasound is used as a trigger for either enzyme catalysis or enzymatic hydrogelation. This approach is highly versatile and can be readily applied to different ion-dependent enzymes or gelation systems. Moreover, this work paves the way for the use of ultrasound as a remote trigger for in vivo hydrogelation.
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