期刊
ADVANCED FUNCTIONAL MATERIALS
卷 30, 期 14, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201909655
关键词
3D cell models; 3D surface-enhanced Raman scattering; depth; imaging; surface-enhanced Raman scattering tags
类别
资金
- European Research Council (ERC) [787510]
- European Commission [731019]
- MINECO [IJCI-2015-24264]
- European Research Council (ERC) [787510] Funding Source: European Research Council (ERC)
Methods to image complex 3D cell cultures are limited by issues such as fluorophore photobleaching and decomposition, poor excitation light penetration, and lack of complementary techniques to verify the 3D structure. Although it remains insufficiently demonstrated, surface-enhanced Raman scattering (SERS) imaging is a promising tool for the characterization of biological complex systems. To this aim, a controllable 3D cell culture model which spans nearly 1 cm(2) in surface footprint is designed. This structure is composed of fibroblasts containing SERS-encoded nanoparticles (i.e., SERS tags), arranged in an alternating layered structure. This sandwich type structure allows monitoring of the SERS signals in the z-axis and with mm dimensions in the xy-axis. Taking advantage of correlative microscopy techniques such as electron microscopy, it is possible to corroborate nanoparticle positioning and distances in z-depths of up to 150 mu m. This study reveals a proof-of-concept method for detailed 3D SERS imaging of a complex, dense 3D cell culture model.
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