期刊
ACTA PHARMACOLOGICA SINICA
卷 41, 期 6, 页码 791-799出版社
NATURE PUBL GROUP
DOI: 10.1038/s41401-019-0345-2
关键词
catalpol; AMPK; mitochondria; biogenesis; type 2 diabetes; skeletal muscle
资金
- National Natural Science Foundation of China [81403154, 81773827, 81573514, 81773995, 81873084, 81573690]
- Scholar of the 14th Batch of Six Talents Peak High-level Talent Selection program [SWYY-094]
- Postgraduate Research Practice Innovation Program of Jiangsu Province [KYCX19-0763]
- Double First-Class University project [CPU2018GY33]
- Qinghai Province High-End Innovative Thousand Talents Program
- Tianshan Cedar Project of the Xinjiang Uygur Autonomous Region [2018XS21]
Mitochondria serve as sensors of energy regulation and glucose levels, which are impaired by diabetes progression. Catalpol is an iridoid glycoside that exerts a hypoglycemic effect by improving mitochondrial function, but the underlying mechanism has not been fully elucidated. In the current study we explored the effects of catalpol on mitochondrial function in db/db mice and C2C12 myotubes in vitro. After oral administration of catalpol (200 mg center dot kg(-1)center dot d(-1)) for 8 weeks, db/db mice exhibited a decreased fasting blood glucose level and restored mitochondrial function in skeletal muscle. Catalpol increased mitochondrial biogenesis, evidenced by significant elevations in the number of mitochondria, mitochondrial DNA levels, and the expression of three genes associated with mitochondrial biogenesis: peroxisome proliferator-activated receptor gammaco-activator 1 (PGC-1 alpha), mitochondrial transcription factor A (TFAM) and nuclear respiratory factor 1 (NRF1). In C2C12 myotubes, catalpol significantly increased glucose uptake and ATP production. These effects depended on activation of AMP-activated protein kinase (AMPK)-mediated mitochondrial biogenesis. Thus, catalpol improves skeletal muscle mitochondrial function by activating AMPK-mediated mitochondrial biogenesis. These findings may guide the development of a new therapeutic approach for type 2 diabetes.
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