期刊
ACTA PARASITOLOGICA
卷 65, 期 2, 页码 317-326出版社
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.2478/s11686-019-00161-6
关键词
Acanthamoeba; Imidazolium ionic liquids; Clinical and environmental isolates; Keratitis; Amoebic encephalitis; In vitro cytotoxicity
资金
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior [001] Funding Source: Medline
Introduction Several strains of the free-living genus Acanthamoeba can cause granulomatous amoebic encephalitis (GAE), a rare chronic and slowly progressive infection of the central nervous system (CNS), and Acanthamoeba keratitis (AK), a sight-threatening eye infectious disease. AK incidence has increased with the popularization of the contact lens wear and its treatment is currently limited and frequently unsuccessful. As imidazolium salts (IS), cationic imidazole derivatives, have promising antimicrobial potential. Materials and Methods The present study evaluated the amoebicidal activity of four IS; 1-n-hexadecyl-3-methylimidazolium methanesulfonate (C(16)MImMeS), chloride (C(16)MImCl) and bis (triluoromethylsulfonyl) imide (C(16)MImNTf2 ), and 1-methyl-3-n-octadecylimidazolium chloride (C(18)MImCl), against the Acanthamoeba castellanii (ATCC30010) environmental strain and a clinical isolate (genotype T4). Results Three IS showed being lethal to 100% of the Acanthamoeba trophozoites at the minimum inhibitory concentrations of 125 and 62.5 mu g/mL (C(16)MImMeS), 31.25 and 62.5 mu g/mL (C(16)MImCl), and 125 and 125 mu g/mL (C(18)MImCl) for ATCC30010 and isolate T4, respectively. C(16)MImNTf2 did not demonstrate amoebicidal activity. All active IS caused the hemolysis of erythrocytes. The cytotoxic effect of the IS was tested in RAW macrophages and human brain microvascular endothelial cells, which demonstrated cytotoxicity in all concentrations tested against both cell lines. As a consequence, these IS with amoebicidal activity presented low selectivity index values (SI) (SI < 1.0), demonstrating lack of parasite selectivity. Conclusion Thus, C(16)MImMeS, C(16)MImCl, and C18MImCl seem to hold greater promise as components for contact lens cleaning and disinfection solutions, instead of direct human application.
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