期刊
ACS CHEMICAL BIOLOGY
卷 15, 期 2, 页码 513-523出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.9b00973
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资金
- University of South Florida
- Shirley W. and William L. Griffin Charitable Foundation
- National Institute of Drug Abuse at the National Institutes of Health [R03-DA034323]
- National Institute of General Medical Science of the National Institutes of Health [R1S-GM107864]
- National Institute of General Medical Sciences [R01-GM115854]
- Mass Spectrometry and Peptide Facility, Department of Chemistry, University of South Florida
- DOE Office of Science [DE-AC02-06CH11357]
- U.S. DOE, Office of Biological and Environmental Research [DE-AC02-06CH11357]
The growing issue of insecticide resistance has meant the identification of novel insecticide targets has never been more important. Arylalkylamine N-acyltransferases (AANATs) have been suggested as a potential new target. These promiscuous enzymes are involved in the N-acylation of biogenic amines to form N-acylamides. In insects, this process is a key step in melanism, hardening of the cuticle, removal of biogenic amines, and in the biosynthesis of fatty acid amides. The unique nature of each AANAT isoform characterized indicates each organism accommodates an assembly of discrete AANATs relatively exclusive to that organism. This implies a high potential for selectivity in insecticide design, while also maintaining polypharmacology. Presented here is a thorough kinetic and structural analysis of AANAT found in one of the most common secondary pests of all plant commodities in the world, Tribolium castaneum. The enzyme, named TcAANAT0, catalyzes the formation of short-chain N-acylarylalkylamines, with short-chain acyl-CoAs (C2-C10), benzoyl-CoA, and succinyl-CoA functioning in the role of acyl donor. Recombinant TcAANAT0 was expressed and purified from E. coli and was used to investigate the kinetic and chemical mechanism of catalysis. The kinetic mechanism is an ordered sequential mechanism with the acyl-CoA binding first. pH-rate profiles and site-directed mutagenesis studies identified amino acids critical to catalysis, providing insights about the chemical mechanism of TcAANAT0. A crystal structure was obtained for TcAANAT0 bound to acetyl-CoA, revealing valuable information about its active site. This combination of kinetic analysis and crystallography alongside mutagenesis and sequence analysis shines light on some approaches possible for targeting TcAANAT0 and other AANATs for novel insecticide design.
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