期刊
ACS CHEMICAL BIOLOGY
卷 15, 期 5, 页码 1127-1133出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.9b00886
关键词
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资金
- National Institutes of Health (NIH) [R01-GM127593]
- National Science Foundation (NSF) [CHE-1708759]
- Pathogenesis of Infectious Disease Award from the Burroughs Wellcome Fund
- NIH Chemistry Biology Interface Training Program [T32-GM071399]
- NSF [MRI-0820996, CHE-1337449]
The bacterial DNA damage response (the SOS response) is a key pathway involved in antibiotic evasion and a promising target for combating acquired antibiotic resistance. Activation of the SOS response is controlled by two proteins: the repressor LexA and the DNA damage sensor RecA. Following DNA damage, direct interaction between RecA and LexA leads to derepression of the SOS response. However, the exact molecular details of this interaction remain unknown. Here, we employ the fluorescent unnatural amino acid acridonylalanine (Acd) as a minimally perturbing probe of the E. coli RecA:LexA complex. Using LexA labeled with Acd, we report the first kinetic model for the reversible binding of LexA to activated RecA. We also characterize the effects that specific amino acid truncations or substitutions in LexA have on RecA:LexA binding strength and demonstrate that a mobile loop encoding LexA residues 75-84 comprises a key recognition interface for RecA. Beyond insights into SOS activation, our approach also further establishes Acd as a sensitive fluorescent probe for investigating the dynamics of protein-protein interactions in other complex systems.
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