期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 15, 页码 17167-17176出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b21075
关键词
DNA nanostructures; immunotherapy; DNA self-assembly; CpG ODNs; immune stimulation
资金
- National Natural Science Foundation of China [81602610, 21874103]
- Fundamental Research Funds for the Central Universities [2042018kf1006, 2042018kf0210]
Unmethylated cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides are immunostimulatory nucleic acids wildly utilized as adjuvants or for vaccines to treat diseases. However, there is a lack of simple and efficient vectors for CpG oligodeoxynucleotide delivery with long-lasting immune stimulation. Herein, self-assembled polymer wires consisting of CpG motifs by hybridization chain reaction were constructed with excellent biocompatibility and immunostimulatory activity. The designed polymer DNA wires acted as programmable multivalent immunoadjuvants and triggered immune response, stimulated pro-inflammatory cytokine secretion, and induced the apoptosis of cancer cells. More strikingly, polymer nanospheres assembled from the polymer DNA wires and cationic poly-L-lysine further improved cellular uptake and continuously stimulate the lysosomal Toll-ike receptor 9 of immune cells, thereby remarkably enhancing the activation of immune cells. These results demonstrated that self-assembled polymer DNA nanoassemblies with multivalent CpG could trigger strong immune response and further induce cancer cell death.
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