期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 5, 页码 5671-5679出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b22234
关键词
liquid biopsy; circulating tumor cells; nanomaterials; aptamer; clonal evolution; nonsmall-cell lung cancer
资金
- National Natural Science Foundation of China [30900650, 81372501, 81572260, 81773299, 81701834, 81502327, 81172232, 31430030]
- Guangdong Natural Science Foundation [2011B031800025, S2012010008378, S2012010008270, S2013010015327, 2013B021800126, 20090171120070, 9451008901002146, 2014A030313052, 2014 J4100132, 2015A020214010, 2016A020215055, 201704020094, 2013B021800259, 2017B070705002, 16ykjc08, 2015ykzd07]
- YFC [2017YFC1308800]
Dynamically monitoring the clonal evolution of lung cancer and performing molecular analyses on tumor cells are challenging but necessary tasks to adjust therapeutic interventions and evaluate treatment efficacy. Circulating tumor cells (CTCs), as a liquid biopsy, may offer an auxiliary tool to identify phenotypic transformation of solid tumors at primary or metastatic sites and uncover their corresponding molecular variation. Herein, we developed an aptamer-modified PEG-PLGA-nanofiber (PPN) microfluidic system optimized for recognizing rare CTC subtypes in lung cancer patients. This unique purification system can be adopted to monitor the clonal evolution of solid tumors by following the intrinsic immunophenotypes of CTCs, while significantly enhancing capture efficiency for polyclonal-derived tumor cells, further facilitating therapeutic evaluation via dynamic CTC enumeration. Combining with downstream single-cell sequencing, the aptamer-modified-PPN microfluidic system was able to provide early insight into tumor heterogeneity and predict histologic transformation in advance, broadening its clinical applications in lung cancer patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据