期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 2, 页码 1997-2008出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b15910
关键词
protein corona; macrophage recognition; immune response; gold nanorods; surface chemistry
资金
- National Basic Research Program of China [2016YFA0201600]
- Key Program for International S&T Cooperation Projects of China [2016YFE0133100]
- Science Fund for Creative Research Groups of the National Natural Science Foundation of China [11621505]
- National Natural Science Foundation of China [31700879]
- CAS Key Research Program for Frontier Sciences [QYZDJ-SSW-SLH022]
- CAS Interdisciplinary Innovation Team
- National Science Fund for Distinguished Young Scholars [11425520]
- Horizon 2020 grant ACEnano [720952]
The significance of protein coronas on the biological fates of nanoparticles has been widely recognized. Therefore, the alterations on biological effects caused by protein coronas need systemic study and interpretation to design novel safe and efficient nanomedicines. In the present study, we present a comprehensive quantitative analysis of the protein coronas on gold nanorods modified with various surface ligands of different chemical compositions and charges. The design of surface ligands is of utmost importance for the functionalization of nanoparticles, and further, the ligand-induced biological identity determines the fate of nano particles in the human body. We found that the surface chemistry influences the composition of the protein corona more profoundly than surface charge. Since the first and most important challenge for administrated nanomedicines is navigating the interaction with macrophages, we further investigated how the surface chemistry-induced specific protein corona affects the phagocytosis and immune responses of macrophages exposed to the corona-nanoparticle complexes. Our results reveal that the protein corona alters the internalization pathways of gold nanorods by macrophages via the interactions of the predominant coronal proteins with specific receptors on the cell membrane. The cytokine secretion profile of macrophages is also highly dependent on the adsorption pattern of the protein corona. The more abundant proteins involved in immune responses, such as acute phase, complement, and tissue leakage proteins, present in the acquired nanoparticle corona, the more macrophage interleukin-1 beta (IL-1 beta) released is stimulated. The ligand-protein corona composition-immune response coefficient analysis may serve next-generation nanomedicines with high efficiency and good safety for better clinical translation.
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