期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 1, 页码 373-379出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b19709
关键词
ratiometric probe; silver nanoclusters; label free; single base mismatch; nucleic acid detection
资金
- National Natural Science Foundation of China [21675093, 21505081, 21974075]
- Natural Science Foundation of Shandong Province of China [ZR2019YQ13]
- Science and Technology Support Plan for Youth Innovation of Colleges and Universities in Shandong Province [2019KJC007]
- Taishan Scholar Program of Shandong Province, China [ts20110829]
A simple and label-free sensing platform with low background based on the chain-displacement triggered self-assembly of Ag NCs was developed for ratiometric visual analysis of intracellular miRNA-21. Based on this sensitively ratiometric sensing approach, a picomole limit detection for miRNA-21 can be obtained. Most importantly, compared with the traditional single base mismatch detection method, our proposed method can realize single base mismatch detection according to the remarkable fluorescence color conversion, rather than simple fluorescence intensity change, which can obviously improve the accuracy and reliability. In addition, successful multicolor real-time monitoring of intracellular miRNA-21 makes the probe a potential candidate for miRNA-21 inhibiting drug screening. Furthermore, MCF-7, HeLa, and normal L02 cells can also be visually differentiated according to the fluorescence color by using the label-free sensing platform, showing its potential prospect in target visual analysis.
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