期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 1, 页码 338-346出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b19246
关键词
Ce-based luminescent metal-organic framework; proprotein convertase subtilisin/kexin type 9; electrochemiluminescence; 4,4',4 '',4 '''-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid); polyamidoamine dendrimer
资金
- National Nature Science Foundation of China [81370403]
- Chongqing foundation [CSTC2015jcyjBX0053]
- Chongqing precision medical key technology research and development and demonstration projects [cstc2016shms-ztzx0042]
- Chongqing medical university scientific research cultivating fund [201414]
- outstanding graduate student cultivation program of Chongqing Medical University [BJRC201928]
We designed a novel luminescent metal-organic framework (MOF) named Ce-TCPP-LMOF (CTM) through a simple one-pot solvothermal method. CTM was synthesized by using the emerging electrochemiluminescent (ECL) material (4,4',4 '',4 '''-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) as the organic ligand and Ce(III) as the metal node. We found that CTM not only has the remarkable ability to emit light but also has a uniform sandwich biscuit shape and suitable nanoscale size, which are promising for further applications. We also applied CTM to construct a novel ECL immunosensor and achieve sensitive detection of the proprotein convertase subtilisin/kexin type 9 (PCSK9), a biomarker related to cardiovascular diseases. To further amplify the ECL signal of CTM, a novel dual-amplified signal strategy was established by inducing a polyamidoamine dendrimer (PAMAM) and gold nanoparticles (AuNPs). Importantly, we first proved that the ECL signal of the CTM/S2O82- system could be enhanced by the PAMAM electric field. As the electron transfer rate was accelerated by the AuNP layer, this ECL signal was further enhanced in AuNP-modified electrodes. The ECL immunosensor showed desirable performance for PCSK9 analysis within a detection range of 50 fg mL(-1) to 10 ng mL(-1) and a low limit of detection of 19.12 +/- 2.69 fg mL(-1). Real sample detection suggested that the immunosensor holds great potential for analyzing clinical serum samples.
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