期刊
ACS APPLIED MATERIALS & INTERFACES
卷 12, 期 11, 页码 12417-12425出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b20639
关键词
biosensor; chemical vapor deposition; SLG/Au NPs; electron beam evaporation; Staphylococcus aureus gene sequence
资金
- National Natural Science Foundation of China [51605060]
- National Key Research and Development Program of China [2016YFB0402702]
- Fundamental Research Funds for the Central Universities [2018CDPTCG0001-5]
- Chongqing Municipality Key Research and Development Program of China [cstc2017rgzn-zdyfX0041]
In this work, a single-layered graphene (SLG)/Au-nanoparticles (NPs)-based Love wave biosensor was prepared for detection of Staphylococcus aureus (S. aureus) gene sequences. The annealing process was proposed to obtain a larger-area interface with ssDNA. The sensitivity was verified by detecting S. aureus gene sequences with a linear detection ranging from 0 to 10 nmol/L, where the limit of detection (LOD) was only 12.4 pg/mL. The stable state of the biosensors based on SLG/Au NPs in the liquid phase could be kept for more than 0.5 h (+/- 0.1 degrees), and the specificity was verified by detecting noncomplementary ssDNA and one-and three-base mismatched ssDNA. The results of our study suggest that a Love wave biosensor based on SLG/Au NPs hold great potential in clinical testing and diagnosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据