期刊
BIOPRESERVATION AND BIOBANKING
卷 13, 期 2, 页码 131-134出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/bio.2014.0071
关键词
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资金
- National Scientific and Technological Research Council
- Brazil (CNPq) [PROTAX 562296/2010-7, 304779/2011-3, 504189/2012-3]
The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis; 6 S. schenckii; 5 S. mexicana, and 3 S. globosa) in saline at 4 degrees C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 degrees C and -80 degrees C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4 degrees C and at -20 degrees C did not alter the number of conidia, but lower conidium counts were observed at -80 degrees C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.
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