期刊
PLANTS-BASEL
卷 8, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/plants8100434
关键词
Camellia sinensis; freezing tolerance; freezing sensitivity; overwintering; photosynthesis system; tea breeding
资金
- National Natural Science Foundation of China [31170643]
- China Agriculture Research System (Tea) [CARS-19]
- Science Technology Department of Zhejiang Province [2752016C02053-5]
Freezing tolerant tea cultivars are urgently needed. The tea cultivars with highly freezing tolerance showed resistance to freezing stress induced photoinhibition. Freezing sensitivity index (H) of 47 tea clonal cultivars was investigated after severe freezing winter in 2016. To develop instrumental methods for freezing tolerance selection, the maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm) and leaf color indicator a on the Hunter color scale were determined on control group (non-frozen) and frozen group (being frozen at -15 degrees C for 2 h and then stood at 20 degrees C for 5 h) of the cultivars. When the two indicators were expressed as the ratios (R-Fv/Fm and R-a) of frozen group to control group, linear regression of the freezing sensitivity index (H) upon the R-Fv/Fm and R-a produced significant relationship respectively, i.e., H = 60.31 - 50.09 R-Fv/Fm (p < 0.01) and H = 30.03 - 10.82 R-a (p < 0.01). Expression of gene psbA encoding D1 protein and gene psbD encoding D2 protein in PSII showed that the frezzing tolerant tea cultivars maintained a high expression level of psbA after freezing stress, which is considered to be beneficial to de novo synthesis of D1 protein and sustaining PSII activity. These findings can provide instrumental tools for assessing freezing tolerance of tea cultivars in tea breeding program.
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