期刊
NANO CONVERGENCE
卷 6, 期 1, 页码 -出版社
SPRINGEROPEN
DOI: 10.1186/s40580-019-0207-0
关键词
Fullerene; Nanoparticle; C-reactive protein; Lateral flow immunochromatographic assay; Fluorescence
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT (MSIT) [NRF-2019R1C1C1006084, NRF-2019R1C1C1006867]
- Center for BioNano Health-Guard - MSIT of Korea as Global Frontier Project [H-GUARD_2013M3A6B2078950]
- Bio and Medical Technology Development Program of the NRF - MSIT of Korea [NRF-2018M3A9E2022821]
- KRIBB Research Initiative Program
- National Research Council of Science & Technology (NST), Republic of Korea [KGM5321911] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1-10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据