期刊
BIOMOLECULES
卷 9, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/biom9100594
关键词
duck hepatitis A virus type 1; insulin-like growth factor-2 mRNA-binding protein-1; 3 ' untranslated region; translation efficiency; viral propagation
资金
- National Natural Science Foundation of China [31772754]
- Shandong Modern Agricultural Technology & Industry System, China [SDAIT-11-15]
- Funds of Shandong Double Tops Program, China [SYL2017YSTD11]
As a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5' untranslated region (UTR), structured sequence elements within the 3' UTR, and a poly(A) tail at the 3' terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3' UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3' UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1.
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