期刊
JOURNAL OF CLINICAL MEDICINE
卷 8, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/jcm8111771
关键词
cartilage lesion; electrical stimulation; capacitively coupled electric field; regenerative medicine; chondrocytes
资金
- German Research Foundation DFG [(CRC 1270 ELAINE)]
Treatment of cartilage lesions remains a clinical challenge. Therefore, biophysical stimuli like electric fields seem to be a promising tool for chondrocytic differentiation and treatment of cartilage lesions. In this in vitro study, we evaluated the effects of low intensity capacitively coupled electric fields with an alternating voltage of 100 mV(RMS) (corresponds to 5.2 x 10(-5) mV/cm) or 1 V-RMS (corresponds to 5.2 x 10(-4) mV/cm) with 1 kHz, on human chondrocytes derived from osteoarthritic (OA) and non-degenerative hyaline cartilage. A reduction of metabolic activity after electrical stimulation was more pronounced in non-degenerative cells. In contrast, DNA contents in OA cells were significantly decreased after electrical stimulation. A difference between 100 mV(RMS) and 1 V-RMS was not detected. However, a voltage-dependent influence on gene and protein expression was observed. Both cell types showed increased synthesis rates of collagen (Col) II, glycosaminoglycans (GAG), and Col I protein following stimulation with 100 mV(RMS), whereas this increase was clearly higher in OA cells. Our results demonstrated the sensitization of chondrocytes by alternating electric fields, especially at 100 mV(RMS), which has an impact on chondrocytic differentiation capacity. However, analysis of further electrical stimulation parameters should be done to induce optimal hyaline characteristics of ex vivo expanded human chondrocytes.
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