期刊
NATURE MICROBIOLOGY
卷 4, 期 12, 页码 2164-2174出版社
NATURE PORTFOLIO
DOI: 10.1038/s41564-019-0568-5
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资金
- Swiss National Science Foundation (SNF) [PMPDP3_158364, PZ00P3_136742]
- Gebert Ruf 'Microbials' programme [GRS-073/17]
- SNF [310030_153074, 310030B_173338/1, Sinergia CRSII_154414/1, NRP 72 407240-167121]
- ETH Zurich [ETH-33 12-2]
- Novartis Freenovation Programme
- Promedica Foundation
- ETH Zurich
- Helmut Horten Foundation
- Boehringer Ingelheim
- Monique Dornonville de la Cour Foundation
- German Research Foundation [STE 1971/4-2 SPP 1656/2]
- German Center for Infection Research (DZIF)
- Swiss National Science Foundation (SNF) [310030_153074, PMPDP3_158364, PZ00P3_136742] Funding Source: Swiss National Science Foundation (SNF)
The microbiota confers colonization resistance, which blocks Salmonella gut colonization(1). As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmonella Typhimurium or Escherichia coli gut colonization and plasmid transfer. Here,we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet. Salmonella gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required Salmonella's AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted Salmonella gut colonization. Both E. coli and Salmonella show much higher bile resistance than the microbiota. Correspondingly, competitive E. coli can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote S. Typhimurium gut infections in mice lacking E. coli in their microbiota. This mouse model may be useful for studying pathogen-microbiota-host interactions, the protective effect of E. coli, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process.
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