期刊
ACS SENSORS
卷 4, 期 12, 页码 3147-3155出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b01268
关键词
selenocysteine; bioluminescence imaging; probe; tumor; sodium selenite
资金
- National Natural Science Foundation of China [21502163, 81673393]
- Jiangsu Natural Science Foundation [BK20150206]
- Foundation for High-Level Talent in Six Areas of Jiangsu Province [2016-YY-038]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX18_2204]
- Jiangsu Overseas Visiting Scholar Program for University Prominent Young & Middle-aged Teachers and Presidents
- Science and Technology Project of Xuzhou [KC19044]
- Jiangsu University Qing Lan Project
- Priority Academic Programme Development of Jiangsu Higher Education Institutions (PAPD)
Selenocysteine (Sec), a vital member of reactive selenium species, is closely implicated in diverse pathophysiological states, including cancer, cardiovascular diseases, diabetes, neurodegenerative diseases, and male infertility. Monitoring Sec in vivo is of significant interest for understanding the physiological roles of Sec and the mechanisms of human diseases associated with abnormal levels of Sec. However, no bioluminescence probe for real-time monitoring of Sec in vivo has been reported. Herein, we present a novel bioluminescent probe BF-1 as an effective tool for the determination of Sec in living cells and in vivo for the first time. BF-1 has advantages of high sensitivity (a detection limit of 8 nM), remarkable bioluminescence enhancement (580 fold), reasonable selectivity, low cytotoxicity, and high signal-to-noise ratio imaging feasibility of Sec in living cells and mice. More importantly, BF-1 affords high sensitivity for monitoring Sec stimulated by Na2SeO3 in tumor-bearing mice. These results demonstrate that our new probe could serve as a powerful tool to selectively monitor Sec in vivo, thus providing a valuable approach for exploring the physiological and pathological functions and anticancer mechanisms of selenium.
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