期刊
ACS SENSORS
卷 4, 期 10, 页码 2771-2777出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b01349
关键词
magnetic biosensors; transverse relaxation rate; Mn(VII)/Mn(II) interconversion; immunomagnetic separation; food-borne pathogen
资金
- National Natural Science Foundation of China [81671784]
- Fundamental Research Funds for Central Universities [2662019PY005]
Paramagnetic ion-mediated sensors can greatly simplify current magnetic sensors for biochemical assays, but it remains challenging because of the limited sensitivity. Herein, we report a magnetic immunosensor relying on Mn(VII)/Mn(II) interconversion and the corresponding change in the low-field nuclear magnetic resonance (LF-NMR) of the transverse relaxation rate (R-2). The fact that the NMR R-2 of the water protons detected in Mn(II) aqueous solution is much stronger than Mn(VII) aqueous solution enables the modulation of the LF-NMR signal intensity of R-2. By employing immunomagnetic separation and enzyme-catalyzed reaction, this Mn(VII)/Mn(II) interconversion allows the development of a background signal-free magnetic immunosensor with a high signal-to-background ratio that enables detection of ractopamine and Salmonella with high sensitivity (the limits of detection for ractopamine and Salmonella are 8.1 pg/mL and 20 cfu/mL, respectively). This Mn-mediated magnetic immunosensor not only retains the good stability but also greatly improves the sensitivity of conventional paramagnetic ion-mediated magnetic sensors, offering a promising platform for sensitive, stable, and convenient bioanalysis.
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