期刊
ACS SENSORS
卷 4, 期 10, 页码 2778-2785出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b01369
关键词
Cardiac troponin I; Electrochemiluminescence; Hybridization chain reaction; Au nanoclusters; Immunosensor
资金
- National Key Research and Development Program of China [2016YFA0201301]
- National Natural Science Foundation of China [21435005, 21627808]
- Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SLH019]
Measurement of cardiac troponin I in the blood is crucial for the early diagnosis of acute myocardial infarction. Herein, a novel and ultrasensitive electrochemiluminescence (ECL) immunosensor has been developed for determination of cardiac troponin I (cTnI) by using Au nanoclusters and hybridization chain reaction (HCR) signal amplification. In this ECL immunosensor, Au nanoclusters were dual-labeled at each end of hairpin DNA (H-1 and H-2) and acted as the luminophore. DNA initiator strands (T-1) and secondary antibody (Ab(2)) were conjugated on Au nano particles (AuNPs) to obtain a smart probe (Ab(2)-AuNP-T-1). In the presence of target cTnI, the sandwiched immunocomplex composed of cTnI, Ab(1), and Ab(2)-AuNP-T-1 was formed. Then the initiator strands T-1 of Ab(2)-AuNP-T-1 opened the hairpin DNA structures and triggered a cascade of hybridization events. Consequently, a large number of Au NCs were indirectly modified on the surface of the electrode, which could react with the coreactant (K2S2O8) and emit a strong ECL signal. Under the optimal conditions, the immunosensor exhibited a wide detection range for cTnI from 5 fg/mL to 50 ng/mL and a low detection limit of 1.01 fg/mL (S/N = 3). Because of the excellent specificity, stability, and reproducibility of the proposed ECL-HCR sensor, it has a great application prospect for cTnI detection in clinical diagnosis.
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