期刊
BMC BIOTECHNOLOGY
卷 16, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12896-016-0297-2
关键词
Tribolium castaneum; Trehalase; RNA interference; Chitin biosynthesis pathway; Gene function
资金
- National Natural Science Foundation of China [31371996, 31672081]
- Visiting Scholar Foundation of State Key Laboratory of Biocontrol at Sun Yat-sen University [SKLBC12F01]
- Hangzhou Science and Technology Development Program of China [20140432B01]
- Program for Excellent Young Teachers in Hangzhou Normal University [JTAS 2011-01-031]
Background: RNA interference is a very effective approach for studies on gene function and may be an efficient method for controlling pests. Trehalase is a key gene in the chitin biosynthesis pathway in insects. Five trehalase genes have been cloned in Tribolium castaneum, though it is not known whether the detailed functions of these trehalases can be targeted for pest control. Results: The functions of all five trehalase genes were studied using RNAi, and the most important results showed that the expression of all 12 genes decreased significantly from 12 to 72 h compared with the control groups, except GP1 at 72 h, when the expression of the TcTre2 gene was suppressed. The results also revealed different abnormal phenotypes, and the observed mortality rates ranged from 17 to 42 %. The qRT-PCR results showed that the expression of TPS, GS, two GP, CHS1a and CHS1b genes decreased significantly, while that of the CHS2 gene decreased or increased after RNAi after the five trehalases were silenced at 48 h. In addition, TPS gene expression decreased from 12 to 72 h after dsTcTre injection. Conclusions: These results demonstrate that silencing of any individual trehalase gene, especially Tre1-4 and Tre2 gene can lead to moulting deformities and a high mortality rate through the regulation of gene expression in the chitin biosynthesis pathway and may be a potential approach for pest control in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据