Polymorphism of Secretary PLA2G2A Gene Associated with Its Serum Level in Type2 Diabetes Mellitus Patients in Northern Iran

Background: Inflammation may occur in Type2 diabetes mellitus. sPLA(2) is among the factors that contribute to the activation of pathways involved in inflammation. Several agents affect serum sPLA(2) level, one of which is genetic diversity. Objective: The current study was performed to determine whether there is a relationship between sPLA(2) gene (-763C > G) polymorphism and circulating sPLA(2) level in patients with Type 2 diabetes. Methods: DNA was extracted from blood samples and used for the amplification of sPLA(2) gene using ARMS-PCR. Results: A statistical analysis using SPSS (version 16) revealed a significant correlation between -763C > G sPLA(2) gene polymorphisms and the disease incidence in patients with T2DM. Among the three possible genotypes (GG, CC, and CG), CG genotype was found to have a higher frequency(53%) in T2DM patients. GG and CC genotypes frequencies were 20 and 27%, respectively. In healthy individuals, the frequencies of CC, GG, and GC genotypes were 77, 9.8% and 13.2%, respectively). Patients with genotype GG had the highest level of sPLA(2). We showed that C>G polymorphism at position-763 is associated with a high level of sPLA(2) in both T2DM patients and healthy individuals. The average of sPLA(2) circulating level was (170.48 +/- 84.90), (106.62 +/- 74.31), in patients and normal individuals, respectively. Conclusion: Our findings show that sPLA(2) serum level is significantly higher in patients with T2DM disease than that in healthy individuals